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  • 生物制劑的制備過程中也需要檢測細菌內(nèi)毒素。生物制劑的種類很多,其中包括菌苗、疫苗、血液制品及干擾素(IFN)等那它們?nèi)绾螜z測細菌內(nèi)毒素呢?一、菌苗菌苗在制備時,多多少少都會受到內(nèi)毒素的污染,尤其是革蘭氏陰性菌菌苗,受內(nèi)毒素污染的程度會更高。...

  • 脂多糖(LPS)作用的靶細胞主要是單核/巨噬細胞。LPS在誘導(dǎo)單核/巨噬細胞合成的分泌細胞因子的過程中脂多糖結(jié)合蛋白(LBP)起著信息轉(zhuǎn)導(dǎo)的作用。血漿中的LBP與LPS的類脂A部分連接形成高親和性復(fù)合物,這一復(fù)合物再經(jīng)膜蛋白CD14受體連接...

  • 一、細胞識別內(nèi)毒素機制脂多糖(LPS)與脂多糖結(jié)合蛋白(LBP)形成復(fù)合物后,能有效地與可溶性CD14(sCD14)或膜結(jié)合CD14(mCD14)受體結(jié)合,并活化細胞反應(yīng)。LPS在細胞膜上的識別在LPS的攝取和誘發(fā)穿膜信號乃至細胞的活化是必...

  • 受體是細胞表面或胞內(nèi)亞細胞組分中的一種蛋白分子,它可以識別并特異地與有生物活性的化學(xué)信號分子(配體)結(jié)合,從而激活或啟動一系列生物化學(xué)反應(yīng),使該化學(xué)信號分子發(fā)揮其特定的生物效應(yīng)。水溶性信號分子通過細胞表面受體起作用,而脂溶性信號分子則主要通...

  • 生命活動的信息是通過信號來傳遞的。多細胞體中,細胞通訊(cellcommunication)包括細胞與細胞之間的通訊(即胞間信號傳遞)和細胞內(nèi)的通訊(即細胞內(nèi)信使傳遞)。細胞間信號傳遞細胞間的信號傳遞有三種形式:(1)通過分泌化學(xué)物質(zhì)作為信...

  • 在《細菌內(nèi)毒素中脂多糖之類脂A化學(xué)結(jié)構(gòu)的簡介》一文中有提到:類脂A(又稱脂質(zhì)A),它是脂多糖的毒性及生物學(xué)活性中心。為一種糖磷脂,現(xiàn)認(rèn)為細菌外膜的外層可能是類脂A。接下來介紹:類脂A及脂多糖的立體構(gòu)型。一、離體脂多糖的雙層結(jié)構(gòu)應(yīng)用X-衍射技...

  • 內(nèi)毒素的主要化學(xué)成分為脂多糖(LPS),脂多糖由結(jié)構(gòu)及生物學(xué)活性互不相同的三部分組成,即O-特異多糖鏈(O-specificpelysaccharidechain)、核心多糖(Coreoligosaccharide)、類脂A(lpidA)。...

  • 類脂A(又稱脂質(zhì)A),系脂多糖的毒性及生物學(xué)活性中心。為一種糖磷脂,現(xiàn)認(rèn)為細菌外膜的外層可能是類脂A。各屬細菌的類脂A結(jié)構(gòu)相似,無種屬特異性,故不同革蘭氏陰性細菌感染時,由內(nèi)毒素引起的毒性作用十分相似。給實驗動物注人類脂A時,可出現(xiàn)典型的內(nèi)...

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